beta-Galactosidase from Escherichia coli, Grade VI, lyophilized powder, 250-600 units/mg protein

Descriptions

Application

?-Galactosidase is conjugated to an antibody that specifically recognizes a target molecule (enzyme immunoassay or EIA). ?-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter.

Biochem/physiol Actions

?-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of ?-galactosidase, in a feedback loop.

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

General description

?-Galactosidase is a tetramer consisting of four equal subunits of 135,000 Da each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.

Packaging

10000 units in poly bottle

1000, 5000 units in glass bottle

Physical form

Partially purified; contains Tris buffer salts, magnesium chloride, DL-dithiothreitol and 2-mercaptoethanol

Physical properties

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Unit Definition

One unit will hydrolyze 1.0 µmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Composition	Form	Price	Quantity
3579573	beta-Galactosidase from Escherichia coli, Grade VI, lyophilized powder, 250-600 units/mg protein	200	SIGMA-ALDRICH	Protein, ≥50% biuret	lyophilized powder	£771.64 (exc VAT) per EACH	- +
3579574	beta-Galactosidase from Escherichia coli, Grade VI, lyophilized powder, 250-600 units/mg protein	200	SIGMA-ALDRICH	Protein, ≥50% biuret	lyophilized powder	£117.00 (exc VAT) per EACH	- +
3579575	beta-Galactosidase from Escherichia coli, Grade VI, lyophilized powder, 250-600 units/mg protein	200	SIGMA-ALDRICH	Protein, ≥50% biuret	lyophilized powder	£345.43 (exc VAT) per EACH	- +