alpha-Chymotrypsin from bovine pancreas, (TLCK treated to inactivate residual tryspin activity), Typ

Descriptions

Analysis Note

Protein determined by A1%/280

Application

?-Chymotrypsin from Sigma has been used to determine the crystal structures of two homologous inhibitors (pars intercerebralis major peptide-C and pars intercerebralis major peptide-D2v) from the insect Locusta migratoria by forming a complex with the enzyme.

Biochem/physiol Actions

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α₁-antitrypsin, α₂-macroglobulin, 10 mM Cu²⁺ and Hg²⁺.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Other Notes

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Packaging

10, 25, 100 mg in glass bottle

Preparation Note

TLCK treatment inactivates trypsin which may be present in chymotrypsin, without affecting the chymotrypsin activity.

Unit Definition

One unit will hydrolyze 1.0 µmole of BTEE per min at pH 7.8 at 25 °C.

Specifications

No detailed specifications are available for this product.

Downloads

There are no downloads for this product.

Code	Description	Quality Level	Manufacturer	Storage Temp.	Solubility	Form	Price	Quantity
3579158	alpha-Chymotrypsin from bovine pancreas, (TLCK treated to inactivate residual tryspin activity), Typ	200	SIGMA-ALDRICH	−20°C	1 mM HCl: soluble 10 mg/mL, clear	essentially salt-free, lyophilized powder	£430.39 (exc VAT) per 100MG	- +
3579159	alpha-Chymotrypsin from bovine pancreas, (TLCK treated to inactivate residual tryspin activity), Typ	200	SIGMA-ALDRICH	−20°C	1 mM HCl: soluble 10 mg/mL, clear	essentially salt-free, lyophilized powder	£85.94 (exc VAT) per 10MG	- +
3579160	alpha-Chymotrypsin from bovine pancreas, (TLCK treated to inactivate residual tryspin activity), Typ	200	SIGMA-ALDRICH	−20°C	1 mM HCl: soluble 10 mg/mL, clear	essentially salt-free, lyophilized powder	£144.86 (exc VAT) per 25MG	- +