alpha-Chymotrypsin-Agarose from bovine pancreas, lyophilized powder, 2,000-3,500 units/g agarose (On

alpha-Chymotrypsin-Agarose from bovine pancreas, lyophilized powder, 2,000-3,500 units/g agarose (On

Analysis Note

Protein determined by A1%/280

Application

α-Chymotrypsin agarose from bovine pancreas has been used to study the purification and characterization of glucoamylase. α-Chymotrypsin agarose from bovine pancreas has also been used in a study to investigate molecular modeling for the design of a biomimetic chimeric ligand.

Biochem/physiol Actions

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.

Serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the bond.

Packaging

50, 100 units in poly bottle

Physical form

Stabilized with lactose

Unit Definition

One unit will hydrolyze 1.0 µmole of N-acetyl-L-tyrosine ethyl ester (ATEE) per min at pH 8.0 at 30 °C.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3579295 alpha-Chymotrypsin-Agarose from bovine pancreas, lyophilized powder, 2,000-3,500 units/g agarose (On 200 SIGMA-ALDRICH −20°C lyophilized powder
£832.93 (exc VAT) per EACH
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3579296 alpha-Chymotrypsin-Agarose from bovine pancreas, lyophilized powder, 2,000-3,500 units/g agarose (On 200 SIGMA-ALDRICH −20°C lyophilized powder
£455.46 (exc VAT) per EACH
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+