alpha-Amylase from porcine pancreas, Type VI-B, >=10 units/mg solid

alpha-Amylase from porcine pancreas, Type VI-B, >=10 units/mg solid

Application

?-Amylase is used to hydrolyze ? bonds of ?-linked polysaccharides, such as starch and glycogen. ?-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis . ?-Amylase, from porcine pancreas, has been used to remove starch during the study of starch metabolism and ripening in tomatoes .

Biochem/physiol Actions

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. The pH optimum is at 7.

General description

We are committed to bringing you?Greener?Alternative Products, which adhere to one or more of The 12 Principles of?Greener?Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Molecular mass: 51-54 kDa.α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

Package size based on α-amylase activity

500000, 1000000, 2500000, 5000000, 10000000 units in poly bottle

Quality

Contains lactose

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

No detailed specifications are available for this product.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3578943 alpha-Amylase from porcine pancreas, Type VI-B, >=10 units/mg solid 200 SIGMA-ALDRICH 2-8°C powder
£930.43 (exc VAT) per EACH
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3578944 alpha-Amylase from porcine pancreas, Type VI-B, >=10 units/mg solid 200 SIGMA-ALDRICH 2-8°C powder
£160.18 (exc VAT) per EACH
-
+
3578945 alpha-Amylase from porcine pancreas, Type VI-B, >=10 units/mg solid 200 SIGMA-ALDRICH 2-8°C powder
£364.93 (exc VAT) per EACH
-
+
3578946 alpha-Amylase from porcine pancreas, Type VI-B, >=10 units/mg solid 200 SIGMA-ALDRICH 2-8°C powder
£75.08 (exc VAT) per EACH
-
+
3578947 alpha-Amylase from porcine pancreas, Type VI-B, >=10 units/mg solid 200 SIGMA-ALDRICH 2-8°C powder
£331.50 (exc VAT) per EACH
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+