Aldolase from rabbit muscle, ammonium sulfate suspension, 10-20 units/mg protein

Aldolase from rabbit muscle, ammonium sulfate suspension, 10-20 units/mg protein

Analysis Note

Protein determined by biuret.

Application

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 .

Aldolase from rabbit muscle has been used: in standard 1-phosphofructokinase from rabbit muscle (RPFK-1) assay as a standard in the characterization of metabolic enzymes from glaucomatous tissuesin fructose 2,6-bisphosphate assay of human cell lines

Biochem/physiol Actions

Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the ?/? 8 barrel fold .

Aldolase interaction with Wiskott-Aldrich syndrome protein (WASP) may modulate actin dynamics. It reverses the inhibition elicited by ascorbate on Muscle-type LDH (LDH-m4).

General description

Aldolase exists as three isoforms in rabbit, which includes type A from muscle, type B from liver and brain associated type C. Aldolases correspond to a molecular weight of 158 kDa and exists as tetramer.

Packaging

5000 units in glass bottle

100, 200, 1000 units in poly bottle

Physical form

Crystalline suspension in 2.5 M (NH4)2SO4, 0.01 M Tris, pH 7.5, 0.001 M EDTA

Unit Definition

One unit will convert 1.0 µmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3579061 Aldolase from rabbit muscle, ammonium sulfate suspension, 10-20 units/mg protein 200 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
£59.20 (exc VAT) per EACH
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3579062 Aldolase from rabbit muscle, ammonium sulfate suspension, 10-20 units/mg protein 200 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
£270.21 (exc VAT) per EACH
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3579063 Aldolase from rabbit muscle, ammonium sulfate suspension, 10-20 units/mg protein 200 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
£80.79 (exc VAT) per EACH
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3579064 Aldolase from rabbit muscle, ammonium sulfate suspension, 10-20 units/mg protein 200 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
£1,055.79 (exc VAT) per EACH
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