Agarose, low gelling temperature, BioReagent, for molecular biology

Analysis Note

The following is a list of properties associated with our agaroses:Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.Gel strength - the force that must be applied to a gel to cause it to fracture.Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Agarose, low gelling temperature has been used:in the single cell gel electrophoresis of glioblastoma multiforme cells for genotoxicity studiesin the preparation of collagen-agarose co-gelsin the embedding of shrimp brain sections

Biochem/physiol Actions

Agarose is used in enzyme immobilization and electrophoresis. It is also used in food and cosmetic industry.

General description

Agarose is a component of cell wall polysaccharides of marine red algae. It has high gelling property and is hydrophilic. It exists in random coil conformation.

Packaging

5, 10, 25, 50, 100, 250 g in poly bottle

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Code Description Pack mp Manufacturer Form Price Quantity
3577329 Agarose, low gelling temperature, BioReagent, for molecular biology 100G ≤65 °C SIGMA-ALDRICH powder
£748.43 (exc VAT) per 100G
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3577330 Agarose, low gelling temperature, BioReagent, for molecular biology 10G ≤65 °C SIGMA-ALDRICH powder
£235.82 (exc VAT) per 10G
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3577331 Agarose, low gelling temperature, BioReagent, for molecular biology 250G ≤65 °C SIGMA-ALDRICH powder
£1,195.71 (exc VAT) per 250G
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3577332 Agarose, low gelling temperature, BioReagent, for molecular biology 25G ≤65 °C SIGMA-ALDRICH powder
£333.25 (exc VAT) per 25G
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3577334 Agarose, low gelling temperature, BioReagent, for molecular biology 5G ≤65 °C SIGMA-ALDRICH powder
£180.46 (exc VAT) per 5G
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