Acylase I from porcine kidney, Grade II, salt-free, lyophilized powder, 500-1,500 units/mg protein

Acylase I from porcine kidney, Grade II, salt-free, lyophilized powder, 500-1,500 units/mg protein

Analysis Note

Protein determined by biuret.

Application

Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids .

Biochem/physiol Actions

Acylase I is a zinc metalloprotein that catalyzes the kinetic resolution of unnatural and rarely occurring ?-amino acids. Its enantioselectivity for the hydrolysis of N-acyl L-?-amino acids is nearly absolute, yet it accepts substrates having a wide range of structure and functionality. Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates.

Packaging

1, 10 g in poly bottle

Unit Definition

One unit will hydrolyze 1.0 µmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3579058 Acylase I from porcine kidney, Grade II, salt-free, lyophilized powder, 500-1,500 units/mg protein 200 SIGMA-ALDRICH −20°C salt-free, lyophilized powder
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3579059 Acylase I from porcine kidney, Grade II, salt-free, lyophilized powder, 500-1,500 units/mg protein 200 SIGMA-ALDRICH −20°C salt-free, lyophilized powder
£242.36 (exc VAT) per 1G
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