Acyl-coenzyme A Synthetase from Pseudomonas sp., >=2 units/mg protein

Descriptions

Application

Acyl-coenzyme A Synthetase may be used to study fatty acid metabolism and lipid metabolism. It has been used to study its interaction with fatty acid transport proteins, which has been found to be involved in the efficient cellular uptake of long-chain fatty acids in adipocytes .

Acyl-coenzyme A (coA) synthetase from Pseudomonas sp. has been used:as ligase in the synthesis of mevalonate derivatives of adenosine triphosphate (ATP)in in vitro fatty acylation assaysin the synthesis of (¹⁴C)Vernoloyl-CoA (Va-CoA),(3) bisphosphonates derivatives of ATP(4) and (³H)Palmitate-CoA

Biochem/physiol Actions

Acyl coenzyme A synthetase proteins are involved in regulating and facilitating long-chain fatty acid transport in mammalian cells .

Acyl-coenzyme A (CoA) synthetase (ACS) enzyme catalyzes a two-step thioesterification reaction involving the conversion of free fatty acids (FAs) to CoA esters. The substrate for ACS varies from two carbon to 26 carbon FAs. It is involved in the activation of FAs for lipid metabolism and enables FA to participate in various cellular metabolic pathways.

General description

Acyl-coenzyme A synthetase belongs to adenylate-forming enzymes superfamily. It has a conserved adenosine triphosphate/adenosine monophosphate (ATP/AMP) binding motif.

Unit Definition

One unit will form 1.0 µmole of AMP and oleoyl coenzyme A from ATP and oleate at pH 8.1 at 25 °C in the presence of Coenzyme A.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price		Quantity
3581652	Acyl-coenzyme A Synthetase from Pseudomonas sp., >=2 units/mg protein	200	SIGMA-ALDRICH	−20°C	powder	Click Add to Quote for price and delivery		- +
3581653	Acyl-coenzyme A Synthetase from Pseudomonas sp., >=2 units/mg protein	200	SIGMA-ALDRICH	−20°C	powder	£305.04 (exc VAT) per EACH		- +