Acetylcholinesterase human, recombinant, expressed in HEK 293 cells, lyophilized powder, >=1,500 uni

Acetylcholinesterase human, recombinant, expressed in HEK 293 cells, lyophilized powder, >=1,500 uni

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Application

Acetylcholinesterase human has been used: as a standard protein to measure protein levels acetylcholinesterase AChE-R and AChE-Sto stimulate human fibroblaststo study its in vitro catalytic activity and to determine the effects of metals, H2O2 and OH radicals on the activity

Biochem/physiol Actions

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.

Acetylcholinesterase(AChE) isregarded as abiomarker inneurotoxicity. It is a modulator ofnitric oxidesignal transduction pathway andmarker of membrane integrityand aging.AChE, hydrolyzes choline esters. It terminates the impulse transmission at cholinergic synapses. AChE does this by rapid hydrolysis of the neurotransmitter acetylcholine (ACh) to acetate and choline. AChE inhibitors prevent the cholinesterase enzyme from breaking down ACh and increases the level and duration of the neurotransmitter action.

General description

Acetylcholinesterase(AChE) is a serine hydrolase, whichbelongs to the carboxyl esterase family of enzymes. AChE is localised at neuromuscular junctions and cholinergic brain synapses.

Packaging

0.1 mg in serum bottle

Physical form

This product is supplied as a lyophilized powder. Lyophilized from 0.22 µm filtered solution in 50mM phosphate buffer pH8.

Unit Definition

One unit will hydrolyze 1.0 µmole of acetylthiocholine to thiocholine and acetate per minute at pH 8.0 at 37 °C.

No detailed specifications are available for this product.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3573308 Acetylcholinesterase human, recombinant, expressed in HEK 293 cells, lyophilized powder, >=1,500 uni 200 SIGMA-ALDRICH −20°C lyophilized powder
£594.75 (exc VAT) per EACH
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3589805 Acetylcholinesterase human, recombinant, expressed in HEK 293 cells, lyophilized powder, >=1,500 uni 200 SIGMA-ALDRICH −20°C lyophilized powder
£1,178.36 (exc VAT) per EACH
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+