5-Fluoroorotic acid hydrate has also been used as a counter-selection agent to screen L. lactis (multi) peptidase knockout mutants and C. albicans. It has been used in synthesis of 5-fluoroorotidine 5?-monophosphate (FOMP).
Useful in the selection of orotidine-5′-phosphate decarboxylase mutants of Saccharomyces cerevisiae.
Biochem/physiol Actions
5-Fluoroorotic acid is a selective inhibitor of rRNA synthesis in mammals.