5-Bromo-4-chloro-3-indolyl beta-D-galactopyranoside, tablet

Descriptions

Application

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

5-Bromo-4-chloro-3-indolyl ?-D-galactopyranoside has been used in the preparation of X-Gal stock solution to add it in LB agar plates for blue-white screening of transformants.

Biochem/physiol Actions

X-gal is routinely used to detect transgene activity in situ.

General description

5-Bromo-4-chloro-3-indolyl β -D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for β-galactosidase.

Quantity

Contains 5 mg substrate per tablet

Reconstitution

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20°C and protected from light. Sterilization is not required.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	InChI key	InChI	Manufacturer	Storage Temp.	Suitability	Form	Price		Quantity
3578178	5-Bromo-4-chloro-3-indolyl beta-D-galactopyranoside, tablet	200	OPIFSICVWOWJMJ-AEOCFKNESA-N	1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1	SIGMA-ALDRICH	−20°C	suitable for identification of lac+ bacterial colonies	tablet	£195.00 (exc VAT) per EACH		- +
3578179	5-Bromo-4-chloro-3-indolyl beta-D-galactopyranoside, tablet	200	OPIFSICVWOWJMJ-AEOCFKNESA-N	1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1	SIGMA-ALDRICH	−20°C	suitable for identification of lac+ bacterial colonies	tablet	£930.43 (exc VAT) per EACH		- +