4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

Application

4-Nitrophenyl phosphate di(tris) salt has been used:as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysatesto determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

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Code Description Quality Level InChI key InChI Manufacturer Storage Temp. Solubility Form Assay Price Quantity
3578555 4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate 100 XXAXKCWOTRABOW-UHFFFAOYSA-N 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2 SIGMA-ALDRICH
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3578556 4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate 100 XXAXKCWOTRABOW-UHFFFAOYSA-N 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2 SIGMA-ALDRICH
£58.22 (exc VAT) per 1G
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3578557 4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate 100 XXAXKCWOTRABOW-UHFFFAOYSA-N 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2 SIGMA-ALDRICH
£640.71 (exc VAT) per 25G
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3578558 4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate 100 XXAXKCWOTRABOW-UHFFFAOYSA-N 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2 SIGMA-ALDRICH
£113.10 (exc VAT) per 5G
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