4-Methylumbelliferyl sulfate potassium salt has been used as a substrate in coupled fluorescence sulfotransferase assay to regenerate 3?-phosphoadenosine 5?-phosphosulfate (PAPS) from 3?-phosphoadenosine 5?-phosphate for cerebroside sulfotransferase (CST) enzyme activity measurements. It has also been used as a substrate in arylsulfatase assay/4-methylumbelliferyl sulfate (4-MUS) assay to test the enzymatic activity of the protein-bead complex.
Biochem/physiol Actions
4-Methylumbelliferyl sulfate (4MUS) is capable of undergoing desulfation and involve in futile cycling with 4MU. It can be utilized to measure the arylsulfatase activity of several sulfatases.
General description
4-Methylumbelliferyl sulfate (4MUS), a general substrate, is a polar metabolite of 4-methylumbelliferone (4MU).