4-Methylumbelliferyl N-acetyl-?-D-glucosaminide has been used as a fluorogenic substrate in the acidic chitinase activity assay. It has also been used to measure the total activity of ?-N-acetylhexosaminidase (NAGase) in water sample filtrates.
Biochem/physiol Actions
4-Methylumbelliferyl-N-acetyl-?-D-glucosaminide (4-MUF-NAG) is a synthetic uncharged fluorogenic substrate for hexosaminidases. It consists of chitin monomer (N-acetyl-?-D-glucosaminide) and 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF). 4-MUF-NAG is used for the detection and estimation of fungal growth by measuring the ?-N-acetylhexosaminidase (NAGase) activity.