3,3,5,5-Tetramethylbenzidine (TMB) Liquid Substrate System, peroxidase substrate

Descriptions

Application

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H₂SO₄, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Caution

This substrate is very light-sensitive and should be protected from direct sunlight or UV sources.

Physical form

Ready-to-use.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price		Quantity
3578799	3,3,5,5-Tetramethylbenzidine (TMB) Liquid Substrate System, peroxidase substrate	300	SIGMA-ALDRICH	2-8°C	liquid	£254.89 (exc VAT) per 100ML		- +
3578800	3,3,5,5-Tetramethylbenzidine (TMB) Liquid Substrate System, peroxidase substrate	300	SIGMA-ALDRICH	2-8°C	liquid	Click Add to Quote for price and delivery		- +