3,3,5,5-Tetramethylbenzidine, >=98.0% (NT)

3,3,5,5-Tetramethylbenzidine, >=98.0% (NT)

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

3,3?,5,5?-Tetramethylbenzidine has been used as a substrate for peroxidase in:enzyme-immunoassay (EIA)indirect competitive enzyme-linked immunosorbent assay (ELISA)sandwich enzyme-linked immunosorbent assay

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Code Description Quality Level mp InChI key InChI Manufacturer Storage Temp. Form Assay Price Quantity
3578010 3,3,5,5-Tetramethylbenzidine, >=98.0% (NT) 300 166-171 °C, 168-171 °C (lit.) UAIUNKRWKOVEES-UHFFFAOYSA-N 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 SIGMA-ALDRICH 2-8°C powder ≥98.0% (NT)
£86.59 (exc VAT) per 1G
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3578011 3,3,5,5-Tetramethylbenzidine, >=98.0% (NT) 300 166-171 °C, 168-171 °C (lit.) UAIUNKRWKOVEES-UHFFFAOYSA-N 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 SIGMA-ALDRICH 2-8°C powder ≥98.0% (NT)
£1,100.64 (exc VAT) per 25G
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3578012 3,3,5,5-Tetramethylbenzidine, >=98.0% (NT) 300 166-171 °C, 168-171 °C (lit.) UAIUNKRWKOVEES-UHFFFAOYSA-N 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 SIGMA-ALDRICH 2-8°C powder ≥98.0% (NT)
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