3, 3,5 ,5-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA, ready to use solution

Descriptions

Application

3, 3?,5 ,5?-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase:to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectantto measure the levels of ?-hemolysin toxinin imaging bound IgGs

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm.

General description

This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	InChI key	InChI	Manufacturer	Storage Temp.	Form	Price		Quantity
3578788	3, 3,5 ,5-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA, ready to use solution	200	UAIUNKRWKOVEES-UHFFFAOYSA-N	1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3	SIGMA-ALDRICH	2-8°C	aqueous solution	£143.46 (exc VAT) per 100ML		- +
3578789	3, 3,5 ,5-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA, ready to use solution	200	UAIUNKRWKOVEES-UHFFFAOYSA-N	1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3	SIGMA-ALDRICH	2-8°C	aqueous solution	£370.07 (exc VAT) per EACH		- +