10X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2

10X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2

Application

10X PCR Buffer without MgCl2 has been used as a component of polymerase chain reaction (PCR) amplification mixture for the mycotoxigenic mould DNA, parasite DNA and Fusarium oxysporum DNA amplification.It has also been used as a component of the PCR mix for the amplification of nuclear ribosomal internal transcribed spacer (ITS2) and the partial ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit rbcl gene for molecular and morphological characterization of Ulva sp from the Persian Gulf.

10× PCR Buffer without MgCl2 has been used with Sigma?s PCR enzymes.

General description

10X PCR Buffer II was tested at a final concentration of 1X (10mM Tris-HCl, pH 8.3 at 25 °C, 50mM KCl), in reactions containing 1-4mM MgCl2, each dNTP at 200 µM, primers defining an approximately 500 base pair region of λ DNA at 1.0µM each, λ DNA template at 1ng/100µL, and Taq DNA polymerase at 2.5 units/100µL. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55 °C to anneal the DNA segments and 72 °C to extend the DNA segments. Following electrophoresis of the reaction products in 1.5% agarose gel, a single band of approximately 500 base pairs was visualized for PCRs containing 1-1.5mM MgCl2.

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Code Description Colour Quality Level Manufacturer Storage Temp. Form Price Quantity
3582398 10X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2 colorless 300 SIGMA-ALDRICH −20°C
£42.55 (exc VAT) per 1.5ML
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3582399 10X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2 colorless 300 SIGMA-ALDRICH −20°C
£165.00 (exc VAT) per 10ML
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3582400 10X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2 colorless 300 SIGMA-ALDRICH −20°C
£112.79 (exc VAT) per 5ML
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