10X PCR Buffer, Optimized for routine PCR with MgCl2 included

10X PCR Buffer, Optimized for routine PCR with MgCl2 included

Application

10X PCR Buffer has been used as a component of the PCR mixture:for the amplification of viral DNAto amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individualsto amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.

Components

Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin

Features and Benefits

Standalone buffer Compatible with JumpStart™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

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Code Description Colour Quality Level Manufacturer Storage Temp. Form Price Quantity
3582394 10X PCR Buffer, Optimized for routine PCR with MgCl2 included colorless 300 SIGMA-ALDRICH −20°C
£81.20 (exc VAT) per EACH
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3582395 10X PCR Buffer, Optimized for routine PCR with MgCl2 included colorless 300 SIGMA-ALDRICH −20°C
£623.46 (exc VAT) per 25ML
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3582396 10x PCR Buffer, Optimized for routine PCR with MgCl2 included colorless 300 SIGMA-ALDRICH −20°C
£255.75 (exc VAT) per 5ML
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3582397 10X PCR Buffer, Optimized for routine PCR with MgCl2 included colorless 300 SIGMA-ALDRICH −20°C
£294.64 (exc VAT) per EACH
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