Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus, lyophilized powder, >=1,000 uni

Descriptions

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.

Biochem/physiol Actions

Used for the release of GPI anchored proteins from the membrane.

General description

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (??) barrel sheets, out of which only six ?? units are found in barrel, lacking helices between strands IV and V and V and VI.

Packaging

5, 25 units in glass bottle

Physical form

Contains phosphate buffer salts, EDTA and stabilizer

Unit Definition

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price		Quantity
3592013	Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus, lyophilized powder, >=1,000 uni	300	SIGMA-ALDRICH	−20°C	lyophilized powder	Click Add to Quote for price and delivery		- +
3592014	Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus, lyophilized powder, >=1,000 uni	300	SIGMA-ALDRICH	−20°C	lyophilized powder	£324.54 (exc VAT) per EACH		- +