Aprotinin from bovine lung, lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell cult

Descriptions

Application

Aprotinin from bovine lung has been used in insulin and glucagon-like peptide-1 assay. It has also been used a in western blotting.

Aprotinin is largely used as an inhibitor of trypsin.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or ﹤3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

Aprotinin is a natural polypeptide of 58 amino acids. It possesses hemostatic/antiplasmin action and helps to reduce bleeding during surgeries. This prevents the necessity of blood transfusion.

Packaging

10, 25 mg in poly bottle

100, 250 mg in glass bottle

Preparation Note

This product is a lyophilized powder that has been cell culture tested. It has an activity of 3-7 TIU/mg.

Unit Definition

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.

When benzoyl-L-arginine ethyl ester (BAEE) is used as substrate one BAPNA unit is approx. 45BAEE µmolar units at pH8.0 at 25°C or approx. 9,000 BAEE A₂₅₃ units at pH7.6 at 25°C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	InChI key	Manufacturer	Product Line	Solubility	Form	Price	Quantity
3589502	Aprotinin from bovine lung, lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell cult	200	ZPNFWUPYTFPOJU-UHFFFAOYSA-N	SIGMA-ALDRICH	BioReagent	H2O: 5 mg/mL	lyophilized powder	Click Add to Quote for price and delivery	- +
3589503	Aprotinin from bovine lung, lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell cult	200	ZPNFWUPYTFPOJU-UHFFFAOYSA-N	SIGMA-ALDRICH	BioReagent	H2O: 5 mg/mL	lyophilized powder	£279.96 (exc VAT) per 10MG	- +
3589504	Aprotinin from bovine lung, lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell cult	200	ZPNFWUPYTFPOJU-UHFFFAOYSA-N	SIGMA-ALDRICH	BioReagent	H2O: 5 mg/mL	lyophilized powder	Click Add to Quote for price and delivery	- +
3589505	Aprotinin from bovine lung, lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell cult	200	ZPNFWUPYTFPOJU-UHFFFAOYSA-N	SIGMA-ALDRICH	BioReagent	H2O: 5 mg/mL	lyophilized powder	£591.96 (exc VAT) per 25MG	- +