Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia), lyophilized powder

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia), lyophilized powder

Analysis Note

Agglutination activity is expressed in µg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Packaging

1, 5 mg in glass bottle

Preparation Note

Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).

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Code Description Quality Level Manufacturer Composition Storage Temp. Impurities Form Price Quantity
3587049 Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia), lyophilized powder 200 SIGMA-ALDRICH Protein, ≥90% biuret 2-8°C
£78.70 (exc VAT) per 1MG
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3587050 Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia), lyophilized powder 200 SIGMA-ALDRICH Protein, ≥90% biuret 2-8°C
£369.11 (exc VAT) per 5MG
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