Trypsin-Agarose, buffered aqueous suspension, trypsin from bovine pancreas

Trypsin-Agarose, buffered aqueous suspension, trypsin from bovine pancreas

Application

An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Physical form

Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative

Preparation Note

TPCK-treated

Unit Definition

One unit will hydrolyze 1.0 µmole of BAEE per min at pH 8.0 at 30?°C (titrimetric assay).

No detailed specifications are available for this product.

There are no downloads for this product.

Code Description Quality Level Manufacturer Matrix Storage Temp. Form Price Quantity
3581423 Trypsin-Agarose, buffered aqueous suspension, trypsin from bovine pancreas 200 SIGMA-ALDRICH beaded agarose 2-8°C
£708.96 (exc VAT) per EACH
-
+
3581424 Trypsin-Agarose, buffered aqueous suspension, trypsin from bovine pancreas 200 SIGMA-ALDRICH beaded agarose 2-8°C
£245.14 (exc VAT) per EACH
-
+
3581425 Trypsin-Agarose, buffered aqueous suspension, trypsin from bovine pancreas 200 SIGMA-ALDRICH beaded agarose 2-8°C
£412.29 (exc VAT) per EACH
-
+