Dulbeccos Modified Eagles Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium

Dulbeccos Modified Eagles Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

General description

This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Reconstitution

Supplement with 0.584 g/L L-glutamine.

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Code Description Quality Level Pack Manufacturer Technique(s) Storage Temp. Impurities Form Price Quantity
3577050 Dulbeccos Modified Eagles Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium 400 500ML SIGMA-ALDRICH cell culture - mammalian: suitable
£35.10 (exc VAT) per 500ML
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3577051 Dulbeccos Modified Eagles Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium - - SIGMA-ALDRICH -
£35.10 (exc VAT) per 500ML
-
+
3577052 Dulbeccos Modified Eagles Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium 400 6X500ML SIGMA-ALDRICH cell culture - mammalian: suitable
£89.61 (exc VAT) per EACH
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