Trehalase from porcine kidney, 0.5-10.0 mg/mL protein basis (BCA), buffered aqueous glycerol solutio

Trehalase from porcine kidney, 0.5-10.0 mg/mL protein basis (BCA), buffered aqueous glycerol solutio

Stock Code: 3581480
Manufacturer Part No: T8778-1UN
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Application


Trehalase has been used:in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leavesin a study to investigate growth arrest by trehalose-6-phosphateto quantify the intracellular trehalose in Lactobacillus casei cells


Biochem/physiol Actions


Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature.


General description


Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification.


Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.


Legal Information


Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow


Physical form


Solution in 50% glycerol containing 1% Triton™ X-100 and 25 mM potassium phosphate, pH 6.5


Unit Definition


One unit will convert 1.0 µmole of trehalose to 2.0 µmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formbuffered aqueous glycerol solution

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