RNase B Glycoprotein Standard from bovine pancreas, Proteomics Grade

Descriptions

Application

RNase B is a preferred substrate with PNGase F for demonstration of N-linked deglycosylation using SDS-PAGE or MALDI-MS. The activity of PNGase F is routinely assayed using RNase B by monitoring the pronounced mobility shift in 12% gels after deglycosylation. Proteomics Grade RNase B has been used as a source of N-glycans following enzymatic digestions and subsequent purification. It has also been used as a glycoprotein standard.

Other Notes

Bovine pancreatic RNase B is a glycoprotein that contains only N-linked glycans. It is a globular protein composed of a single domain that occurs naturally as a lesser component in mixture along with ribonuclease A (RNase A), which is the non-glycosylated core form. RNase B contains a single glycosylation site at Asn³⁴ at which from five to nine mannose residues are attached to the chitobiose core, i.e. Man_5-9GlcNAc₂. Due to the heterogeneity in the glycosylation at Asn³⁴, RNase B exists as five glycosylated varients, with a molecular weight of approx. 15 kDa.

Quality

RNAse B Glycoprotein Standard has been highly purified to remove contaminating RNase A.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Assay	Price		Quantity
3578724	RNase B Glycoprotein Standard from bovine pancreas, Proteomics Grade	200	SIGMA-ALDRICH	−20°C	lyophilized powder	≥90% (SDS-PAGE)	£702.00 (exc VAT) per 1MG		- +
3578725	RNase B Glycoprotein Standard from bovine pancreas, Proteomics Grade	200	SIGMA-ALDRICH	−20°C	lyophilized powder	≥90% (SDS-PAGE)	£434.57 (exc VAT) per 500UG		- +