Ribonuclease T1 from Aspergillus oryzae, ammonium sulfate suspension, 300,000-600,000 units/mg prote

Ribonuclease T1 from Aspergillus oryzae, ammonium sulfate suspension, 300,000-600,000 units/mg prote

Analysis Note

Protein determined by E1%/280

Application

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .

Biochem/physiol Actions

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3’;-phosphate group of a guanidine ribonucleotide and 5’;-hydroxyl of the adjacent nucleotide. The initial product is a 2’;:3’; cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3’;-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.

Physical form

Suspension in 2.8 M (NH4)2SO4 solution

Unit Definition

One unit will produce acid soluble oligonucleotides equivalent to a δA260 of 1.0 in 15 min at pH?7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3581251 Ribonuclease T1 from Aspergillus oryzae, ammonium sulfate suspension, 300,000-600,000 units/mg prote 200 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
£116.72 (exc VAT) per EACH
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+
3581252 Ribonuclease T1 from Aspergillus oryzae, ammonium sulfate suspension, 300,000-600,000 units/mg prote 200 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
£500.04 (exc VAT) per EACH
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+