Phospholipase A2 from porcine pancreas, ammonium sulfate suspension, >=600 units/mg protein

Phospholipase A2 from porcine pancreas, ammonium sulfate suspension, >=600 units/mg protein

Analysis Note

Protein determined by biuret.

Application

Phospholipase A2 has been used in phospholipase assay and to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation.

Biochem/physiol Actions

It has a high catalytic activity on aggregated substrates compared to monomeric substrates.

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

General description

Phospholipase A2 is a small disulfide-rich protein having 124 residues. It is a calcium-dependent enzyme.

Packaging

1, 5, 10 mg in glass bottle

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 5.5

Unit Definition

One unit will hydrolyze 1.0 µmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 at 37 °C.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3592018 Phospholipase A2 from porcine pancreas, ammonium sulfate suspension, >=600 units/mg protein 200 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
£429.00 (exc VAT) per EA
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3592019 Phospholipase A2 from porcine pancreas, ammonium sulfate suspension, >=600 units/mg protein 200 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
£75.08 (exc VAT) per 1MG
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3592020 Phospholipase A2 from porcine pancreas, ammonium sulfate suspension, >=600 units/mg protein 200 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
£260.46 (exc VAT) per 5MG
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