beta-Galactosidase from Escherichia coli, aqueous glycerol suspension, 600-1,200 units/mg protein (b

Descriptions

Application

β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Other Notes

Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).

Packaging

5000 units in glass bottle

Physical form

Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4

Physical properties

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Unit Definition

One unit will hydrolyze 1.0 µmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price	Quantity
3579532	beta-Galactosidase from Escherichia coli, aqueous glycerol suspension, 600-1,200 units/mg protein (b	200	SIGMA-ALDRICH	−20°C	aqueous glycerol suspension	£153.21 (exc VAT) per EACH	- +
3579533	beta-Galactosidase from Escherichia coli, aqueous glycerol suspension, 600-1,200 units/mg protein (b	200	SIGMA-ALDRICH	−20°C	aqueous glycerol suspension	£376.07 (exc VAT) per EACH	- +
3579534	beta-Galactosidase from Escherichia coli, aqueous glycerol suspension, 600-1,200 units/mg protein (b	200	SIGMA-ALDRICH	−20°C	aqueous glycerol suspension	£534.86 (exc VAT) per EACH	- +