?-Glycerophosphate Dehydrogenase from rabbit muscle has been used:in the reaction mixture to measure the glycerol kinase activityto demonstrate compartmentalized enzymatic reactions where NADH is involvedin the reaction mixture assay for L-fuculose-1-phosphate aldolase
Biochem/physiol Actions
?-Glycerophosphate dehydrogenase catalyzes the oxidation of L-?-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.
General description
?-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.
Physical form
Crystalline suspension in 3.2 M (NH4)2SO4 and 0.1 g/L EDTA solution, pH 6.0
Preparation Note
Prepared by modification of the method of Fondy.
Unit Definition
One unit will convert 1.0 µmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.